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. 2021 Feb 1;10:e59551. doi: 10.7554/eLife.59551

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© 2021, Young et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (A) Configuration of experiments comparing strength of primary visual cortex (V1) FF input to pairs of L6 looped and non-looped IT neurons in lateral visual area (V2L) or medial visual area (V2M). (B) Example pair of subcellular channelrhodopsin-2 (ChR2)-assisted circuit mapping (sCRACM) maps overlaid on reconstructed dendrites showing monosynaptic V1 FF inputs to a looped IT neuron (left) and an adjacent non-looped IT neuron (right) recorded in V2L. (C) Left, paired comparisons of perisomatic FF input to looped vs. non-looped IT neurons (n, number of cell pairs; N, number of mice); black dots, V1→V2L inputs; gray dots, V1→V2M inputs. Traces were generated by averaging the mean perisomatic excitatory postsynaptic current (EPSC) of each neuron across all neurons in the same projection class. Colors correspond to (A). Blue tick, laser pulse. Scale bars in all panels, 2 pA and 20 ms. Right, sCRACM Response Index (SRI) of the same data. Number of cell pairs and animals are the same as in the left plot unless otherwise specified. Horizontal line, mean. *, p<0.05, see text for exact value. (D) Same as C for apical inputs (SRI: V1→V2L, n = 7, N = 6; V1→V2M, n = 7, N = 6). (E) Configuration of experiment comparing strength of V1 FF input to pairs of L6 looped IT and CT neurons in V2L. (F) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V1 FF inputs to a looped IT neuron (left) and an adjacent CT neuron (right) recorded in V2L. (G) Paired comparisons and SRI of perisomatic FF input to looped IT vs. CT neurons. (H) Paired comparisons and SRI (n = 5, N = 5) of apical FF input to looped IT vs. CT neurons. (I) Configuration of experiments comparing strength of V2L or V2M FB input to pairs of L6 looped and non-looped IT neurons in V1. (J) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V2L FB inputs to a looped IT neuron (left) and an adjacent non-looped IT neuron (right) recorded in V1. (K) Paired comparisons and SRI of perisomatic FB input to looped vs. non-looped IT neurons. Dark green dots, V2L→V1 inputs; light green dots, V2M→V1 inputs. (L) Paired comparisons and SRI (V2L→V1, n = 5, N = 5; V2M→V1, n = 4, N = 4) of apical FB input to looped vs. non-looped IT neurons. (M) Configuration of experiment comparing strength of V2L FB input to pairs of L6 looped IT and CT neurons in V1. (N) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V2L FB inputs to a looped IT neuron (left) and an adjacent CT neuron (right) recorded in V1. (O) Paired comparisons and SRI of perisomatic FB input to looped IT vs. CT neurons. (P) Paired comparisons and SRI (n = 8, N = 7) of apical FB input to looped IT vs. CT neurons.

Figure 4—figure supplement 1. — (A) Configuration of experiments comparing strength of primary visual cortex (V1) FF input to pairs of L6 looped and non-looped IT neurons in lateral visual area (V2L) or medial visual area (V2M). (B) Example pair of subcellular channelrhodopsin-2 (ChR2)-assisted circuit mapping (sCRACM) maps overlaid on reconstructed dendrites showing monosynaptic V1 FF inputs to a looped IT neuron (left) and an adjacent non-looped IT neuron (right) recorded in V2L. (C) Left, paired comparisons of perisomatic FF input to looped vs. non-looped IT neurons (n, number of cell pairs; N, number of mice); black dots, V1→V2L inputs; gray dots, V1→V2M inputs. Traces were generated by averaging the mean perisomatic excitatory postsynaptic current (EPSC) of each neuron across all neurons in the same projection class. Colors correspond to (A). Blue tick, laser pulse. Scale bars in all panels, 2 pA and 20 ms. Right, sCRACM Response Index (SRI) of the same data. Number of cell pairs and animals are the same as in the left plot unless otherwise specified. Horizontal line, mean. *, p<0.05, see text for exact value. (D) Same as C for apical inputs (SRI: V1→V2L, n = 7, N = 6; V1→V2M, n = 7, N = 6). (E) Configuration of experiment comparing strength of V1 FF input to pairs of L6 looped IT and CT neurons in V2L. (F) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V1 FF inputs to a looped IT neuron (left) and an adjacent CT neuron (right) recorded in V2L. (G) Paired comparisons and SRI of perisomatic FF input to looped IT vs. CT neurons. (H) Paired comparisons and SRI (n = 5, N = 5) of apical FF input to looped IT vs. CT neurons. (I) Configuration of experiments comparing strength of V2L or V2M FB input to pairs of L6 looped and non-looped IT neurons in V1. (J) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V2L FB inputs to a looped IT neuron (left) and an adjacent non-looped IT neuron (right) recorded in V1. (K) Paired comparisons and SRI of perisomatic FB input to looped vs. non-looped IT neurons. Dark green dots, V2L→V1 inputs; light green dots, V2M→V1 inputs. (L) Paired comparisons and SRI (V2L→V1, n = 5, N = 5; V2M→V1, n = 4, N = 4) of apical FB input to looped vs. non-looped IT neurons. (M) Configuration of experiment comparing strength of V2L FB input to pairs of L6 looped IT and CT neurons in V1. (N) Example pair of sCRACM maps overlaid on reconstructed dendrites showing monosynaptic V2L FB inputs to a looped IT neuron (left) and an adjacent CT neuron (right) recorded in V1. (O) Paired comparisons and SRI of perisomatic FB input to looped IT vs. CT neurons. (P) Paired comparisons and SRI (n = 8, N = 7) of apical FB input to looped IT vs. CT neurons.