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. 2021 Feb 1;10:e59551. doi: 10.7554/eLife.59551

Figure 8. Feedback (FB) inputs in L6 can selectively modulate activity in looped intratelencephalic (IT) neurons.

Figure 8.

(A) Experiment configuration. In the absence of channel blockers, V2M→V1 FB axons were photostimulated using an LED during current-clamp recordings of looped and non-looped IT neurons in L6. (B) Example of excitatory postsynaptic potentials (EPSPs) from an example pair. Blue shade, light pulse. (C) Left, paired comparisons of EPSP amplitudes evoked in looped vs. non-looped IT neurons. Inset traces represent group averages for each projection class. Blue shade, light pulse. Right, CRACM Response Index (CRI) of the same data (n = 10, N = 7). (D) Example traces of FB modulation in a looped IT neuron. Cells were driven by a sustained positive current injection. Black traces, LED OFF trials; blue traces, LED ON trials. Blue bar, duration of the LED light pulse. Gray shading, time period used to analyze spiking activity in (E). (E) Spike rate difference between LED-ON and LED-OFF trials in looped and non-looped IT neurons as a function of the amount of current injected during the depolarization step. *, p=0.0273, paired t-test with Bonferroni correction for seven comparisons.