Key resources table.
Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
---|---|---|---|---|
Antibody | Anti-mouse CD3ε (clone# 145–2 C11) FITC, PE, and BV421 | Biolegend | 100305/07/35 | Dilution: 1:300 |
Antibody | Anti-mouse/human B220 (clone# RA3-6B2) APC/Cy7 and A700 | Biolegend | 103223/31 | Dilution: 1:750 |
Antibody | Anti-mouse CD19 (clone# 6D5) APC/Cy7 | Biolegend | 115529 | Dilution: 1:750 |
Antibody | Anti-mouse I-Ab (clone# AF6-120.1) APC and PerCP/Cy5.5 | Biolegend | 116417/15 | Dilution: 1:750 |
Antibody | Anti-mouse CD11c (clone# N418) PE/Cy7 and BV605 | Biolegend | 117317/33 | Dilution: 1:750 |
Antibody | Anti-mouse/human CD11b (clone# M1/70) BV711 | Biolegend | 101241 | Dilution: 1:750 |
Antibody | Anti-mouse CD103 (clone# 2E7) BV421 | Biolegend | 121421 | Dilution: 1:300 |
Antibody | Anti-mouse CD4 (clone# RM4-5) BV711, PE, and PB | Biolegend | 100549/11/34 | Dilution: 1:750 |
Antibody | Anti-mouse CD25 (clone# PC61) APC, BV605, PerCP/Cy5.5, and PE-Cy7 | Biolegend | 102011/35/29/15 | Dilution: 1:750 |
Antibody | Anti-mouse/human CD44 (clone# IM7) APC/Cy7 and BV605 | Biolegend | 103027/47 | Dilution: 1:750 |
Antibody | Anti-mouse CD62L (clone# MEL-14) APC/Cy7 and BV605 | Biolegend | 104427/37 | Dilution: 1:750 |
Antibody | Anti-mouse FOXP3 (clone# FJK-16s) FITC | Thermo Fisher | 11-5773-82 | Dilution: 1:200 |
Antibody | Anti-mouse GATA3 (clone# 16E10A23) PE | Biolegend | 653803 | Dilution: 1:30 |
Antibody | Anti-mouse RORγt (clone# B2D) APC | Thermo Fisher | 17-6981-80 | Dilution: 1:200 |
Antibody | Anti-mouse TBET (clone# 4B10) PE/Cy7 | Biolegend | 644823 | Dilution: 1:200 |
Antibody | Anti-mouse HELIOS (clone# 22F6) A647 | Biolegend | 137208 | Dilution: 1:200 |
Antibody | Anti-mouse CXCR3 (clone# CXCR3-173) BV421 | Biolegend | 126521 | Dilution: 1:300 |
Antibody | Anti-mouse Thy1.1 (clone# 30-H12) PE/Cy7 | Biolegend | 105325 | Dilution: 1:750 |
Antibody | Anti-mouse LAP (clone# TW7-16B4) BV421 | Biolegend | 141407 | Dilution: 1:100 |
Antibody | Anti-mouse CD51 (clone# RMV-7) PE | Biolegend | 104105 | Dilution: 1:200 |
Antibody | Anti-mouse CD80 (clone# 16-10A1) APC | Biolegend | 104713 | Dilution: 1:750 |
Antibody | Anti-mouse CD86 (clone# GL-1) BV605 | Biolegend | 105037 | Dilution: 1:300 |
Antibody | Anti-mouse CD40 (clone# 3/23) APC | Biolegend | 124611 | Dilution: 1:750 |
Antibody | Anti-mouse CD273 (clone# TY25) APC | Biolegend | 107210 | Dilution: 1:300 |
Antibody | Anti-mouse CD274 (clone# 10F.9G2) BV421 | Biolegend | 124315 | Dilution: 1:300 |
Antibody | Anti-mouse XCR1 (clone# ZET) APC | Biolegend | 148205 | Dilution: 1:300 |
Antibody | Anti-mouse CD36 (clone# CRF D-2712) PE | Becton Dickinson | 562702 | Dilution: 1:200 |
Antibody | Anti-mouse CD45.1 (clone# A20) PE, APC, and PE/Cy7 | Biolegend | 110707/14/29 | Dilution: 1:750 |
Antibody | Anti-mouse CD45.2 (clone# 104) PE, APC, PE/Cy7, and A700 | Biolegend | 109807/14/29/21 | Dilution: 1:750 |
Antibody | Anti-mouse VB6 (clone# RR4-7) PE and APC | Biolegend | 140003/5 | Dilution: 1:750 |
Antibody | Anti-mouse Va2 (clone# B20.1) APC/Cy7 and PerCP/Cy5.5 | Biolegend | 127818/13 | Dilution: 1:750 |
Antibody | Anti-mouse VB5 (clone# MR9-4) PE | Biolegend | 139503 | Dilution: 1:750 |
Antibody | Anti-mouse F4/80 (clone# BM8) PE/Cy7 | Biolegend | 123113 | Dilution: 1:750 |
Antibody | Anti-mouse SIRPα (clone# P84) A700 | Biolegend | 144021 | Dilution: 1:750 |
Antibody | Anti-mouse CD24 (clone# M1/69) PE | Biolegend | 101807 | Dilution: 1:750 |
Antibody | Anti-mouse CD101 (clone# Moushi101) PE/Cy7 | Thermo Fisher | 50-112-3316 | Dilution: 1:300 |
Strain, strain background (Mus musculus) | OT-II TCR transgenic mice | The Jackson Laboratory (JAX) | #004194 | |
Strain, strain background (Mus musculus) | Ccr7GFP knockin/knockout mice | The Jackson Laboratory (JAX) | #027913 | |
Strain, strain background (Mus musculus) | Itgax-Cre mice | The Jackson Laboratory (JAX) | #008068 | |
Strain, strain background (Mus musculus) | Irf4fl/fl mice | The Jackson Laboratory (JAX) | #009380 | |
Strain, strain background (Mus musculus) | CT2 TCR transgenic mice | Nutsch et al., 2016 | ||
Strain, strain background (Mus musculus) | CT6 TCR transgenic mice | Nutsch et al., 2016 | ||
Strain, strain background (Mus musculus) | Rag1–/– mice | The Jackson Laboratory (JAX) | #002216 | |
Strain, strain background (Mus musculus) | Foxp3IRES-GFPmice | The Jackson Laboratory (JAX) | #006772 | |
Strain, strain background (Mus musculus) | Foxp3IRES-Thy1.1mice | Liston et al., 2008 | ||
Strain, strain background (Mus musculus) | Irf8Δ149en/Δ149en mice; formerly Irf8 +32 5’–/– | Durai et al., 2019 | ||
Strain, strain background (Mus musculus) | Batf3–/– mice | Hildner et al., 2008 | ||
Strain, strain background (Mus musculus) | Zbtb46GFP mice | Satpathy et al., 2012 | ||
Strain, strain background (Mus musculus) | CD207-DTA mice; formerly huLangerin-DTA | Kaplan et al., 2005 |