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. 2021 Feb 3;10:e54792. doi: 10.7554/eLife.54792

Key resources table.

Reagent type
(species) or
resource
Designation Source or
reference
Identifiers Additional
information
Antibody Anti-mouse CD3ε (clone# 145–2 C11) FITC, PE, and BV421 Biolegend 100305/07/35 Dilution: 1:300
Antibody Anti-mouse/human B220 (clone# RA3-6B2) APC/Cy7 and A700 Biolegend 103223/31 Dilution: 1:750
Antibody Anti-mouse CD19 (clone# 6D5) APC/Cy7 Biolegend 115529 Dilution: 1:750
Antibody Anti-mouse I-Ab (clone# AF6-120.1) APC and PerCP/Cy5.5 Biolegend 116417/15 Dilution: 1:750
Antibody Anti-mouse CD11c (clone# N418) PE/Cy7 and BV605 Biolegend 117317/33 Dilution: 1:750
Antibody Anti-mouse/human CD11b (clone# M1/70) BV711 Biolegend 101241 Dilution: 1:750
Antibody Anti-mouse CD103 (clone# 2E7) BV421 Biolegend 121421 Dilution: 1:300
Antibody Anti-mouse CD4 (clone# RM4-5) BV711, PE, and PB Biolegend 100549/11/34 Dilution: 1:750
Antibody Anti-mouse CD25 (clone# PC61) APC, BV605, PerCP/Cy5.5, and PE-Cy7 Biolegend 102011/35/29/15 Dilution: 1:750
Antibody Anti-mouse/human CD44 (clone# IM7) APC/Cy7 and BV605 Biolegend 103027/47 Dilution: 1:750
Antibody Anti-mouse CD62L (clone# MEL-14) APC/Cy7 and BV605 Biolegend 104427/37 Dilution: 1:750
Antibody Anti-mouse FOXP3 (clone# FJK-16s) FITC Thermo Fisher 11-5773-82 Dilution: 1:200
Antibody Anti-mouse GATA3 (clone# 16E10A23) PE Biolegend 653803 Dilution: 1:30
Antibody Anti-mouse RORγt (clone# B2D) APC Thermo Fisher 17-6981-80 Dilution: 1:200
Antibody Anti-mouse TBET (clone# 4B10) PE/Cy7 Biolegend 644823 Dilution: 1:200
Antibody Anti-mouse HELIOS (clone# 22F6) A647 Biolegend 137208 Dilution: 1:200
Antibody Anti-mouse CXCR3 (clone# CXCR3-173) BV421 Biolegend 126521 Dilution: 1:300
Antibody Anti-mouse Thy1.1 (clone# 30-H12) PE/Cy7 Biolegend 105325 Dilution: 1:750
Antibody Anti-mouse LAP (clone# TW7-16B4) BV421 Biolegend 141407 Dilution: 1:100
Antibody Anti-mouse CD51 (clone# RMV-7) PE Biolegend 104105 Dilution: 1:200
Antibody Anti-mouse CD80 (clone# 16-10A1) APC Biolegend 104713 Dilution: 1:750
Antibody Anti-mouse CD86 (clone# GL-1) BV605 Biolegend 105037 Dilution: 1:300
Antibody Anti-mouse CD40 (clone# 3/23) APC Biolegend 124611 Dilution: 1:750
Antibody Anti-mouse CD273 (clone# TY25) APC Biolegend 107210 Dilution: 1:300
Antibody Anti-mouse CD274 (clone# 10F.9G2) BV421 Biolegend 124315 Dilution: 1:300
Antibody Anti-mouse XCR1 (clone# ZET) APC Biolegend 148205 Dilution: 1:300
Antibody Anti-mouse CD36 (clone# CRF D-2712) PE Becton Dickinson 562702 Dilution: 1:200
Antibody Anti-mouse CD45.1 (clone# A20) PE, APC, and PE/Cy7 Biolegend 110707/14/29 Dilution: 1:750
Antibody Anti-mouse CD45.2 (clone# 104) PE, APC, PE/Cy7, and A700 Biolegend 109807/14/29/21 Dilution: 1:750
Antibody Anti-mouse VB6 (clone# RR4-7) PE and APC Biolegend 140003/5 Dilution: 1:750
Antibody Anti-mouse Va2 (clone# B20.1) APC/Cy7 and PerCP/Cy5.5 Biolegend 127818/13 Dilution: 1:750
Antibody Anti-mouse VB5 (clone# MR9-4) PE Biolegend 139503 Dilution: 1:750
Antibody Anti-mouse F4/80 (clone# BM8) PE/Cy7 Biolegend 123113 Dilution: 1:750
Antibody Anti-mouse SIRPα (clone# P84) A700 Biolegend 144021 Dilution: 1:750
Antibody Anti-mouse CD24 (clone# M1/69) PE Biolegend 101807 Dilution: 1:750
Antibody Anti-mouse CD101 (clone# Moushi101) PE/Cy7 Thermo Fisher 50-112-3316 Dilution: 1:300
Strain, strain background (Mus musculus) OT-II TCR transgenic mice The Jackson Laboratory (JAX) #004194
Strain, strain background (Mus musculus) Ccr7GFP knockin/knockout mice The Jackson Laboratory (JAX) #027913
Strain, strain background (Mus musculus) Itgax-Cre mice The Jackson Laboratory (JAX) #008068
Strain, strain background (Mus musculus) Irf4fl/fl mice The Jackson Laboratory (JAX) #009380
Strain, strain background (Mus musculus) CT2 TCR transgenic mice Nutsch et al., 2016
Strain, strain background (Mus musculus) CT6 TCR transgenic mice Nutsch et al., 2016
Strain, strain background (Mus musculus) Rag1–/– mice The Jackson Laboratory (JAX) #002216
Strain, strain background (Mus musculus) Foxp3IRES-GFPmice The Jackson Laboratory (JAX) #006772
Strain, strain background (Mus musculus) Foxp3IRES-Thy1.1mice Liston et al., 2008
Strain, strain background (Mus musculus) Irf8Δ149en/Δ149en mice; formerly Irf8 +32 5’–/– Durai et al., 2019
Strain, strain background (Mus musculus) Batf3–/– mice Hildner et al., 2008
Strain, strain background (Mus musculus) Zbtb46GFP mice Satpathy et al., 2012
Strain, strain background (Mus musculus) CD207-DTA mice; formerly huLangerin-DTA Kaplan et al., 2005