Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (D. melanogaster) | y[1]w[*] | Pfeiffer et al., 2008 | ||
| Genetic reagent (D. melanogaster) | UAS-shibirets1 | Pfeiffer et al., 2012 | FLYB: FBst0066600; RRID:BDSC_66600 | |
| Genetic reagent (D. melanogaster) | LexAop-GCaMP6s, UAS-Chrimson | Allan Wong (Janelia Research Campus) | N/A | 13xLexAop2-Syn21-opGCaMP6s in su(Hw)attP8, 10xUAS-Syn21-Chrimson88-tdTomato-3.1 in attP18 |
| Genetic reagent (D. melanogaster) | trans-Tango | Talay et al., 2017 | FLYB: FBst0077124; RRID:BDSC_ 77124 |
trans-Tango in attP40 |
| Genetic reagent (D. melanogaster) | UAS-myrGFP, QUAS-mtdTomato | Talay et al., 2017 | FLYB: FBst0077479; RRID:BDSC_77479 | 10xUAS-myrGFP, 5xQUAS-mtdTomato(3xHA) in su(Hw)attP8 |
| Genetic reagent (D. melanogaster) | UAS-CD2, QUAS-mtdTomato | This study | N/A | 10xUAS-CD2, 5xQUAS-mtdTomato(3xHA) in su(Hw)attP8 |
| Genetic reagent (D. melanogaster) | brp-SNAP | Kohl et al., 2014 | FLYB: FBst0058397; RRID:BDSC_ 58397 |
brp[SNAPf-tag]/Cyo |
| Genetic reagent (D. melanogaster) | LexAop-GFP | Pfeiffer et al., 2010 | FLYB: FBst0032203; RRID:BDSC_32203 |
13XLexAop2-mCD8::GFP in attP2 |
| Genetic reagent (D. melanogaster) | MB002B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135053 RRID:BDSC_68305 |
MBON β′2mp (4), γ5β′2a (2) |
| Genetic reagent (D. melanogaster) | MB011B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135062 RRID:BDSC_68294 |
MBON γ5β′2a (4), β′2mp (3), β′2mp_bilateral (3) |
| Genetic reagent (D. melanogaster) | MB018B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135069 RRID:BDSC_68296 |
MBON α′2 (4) |
| Genetic reagent (D. melanogaster) | MB026B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135077 RRID:BDSC_68300 |
MBON α′1 (3), α′3ap (2) |
| Genetic reagent (D. melanogaster) | MB027B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135078 RRID:BDSC_68301 |
MBON α′3ap (5), α′3 m (5) |
| Genetic reagent (D. melanogaster) | MB050B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135100 RRID:BDSC_68365 |
MBON α′1 (2), α2sc (4) |
| Genetic reagent (D. melanogaster) | MB051B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135101 RRID:BDSC_68275 |
MBON α′2 (1), γ2α′1 (4) |
| Genetic reagent (D. melanogaster) | MB051C-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135136 RRID:BDSC_68249 |
MBON α′2 (1), γ2α′1 (3) |
| Genetic reagent (D. melanogaster) | MB057B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135106 RRID:BDSC_68277 |
MBON β′1 (3) |
| Genetic reagent (D. melanogaster) | MB074C-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135122 RRID:BDSC_68282 |
MBON β′2mp (4), β2β′2a (3), γ5β′2a (1) |
| Genetic reagent (D. melanogaster) | MB077B- split-GAL4 | Aso et al., 2014a | RRID:BDSC_68283 | MBON γ2α′1 (4) |
| Genetic reagent (D. melanogaster) | MB077C- split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135125 RRID:BDSC_68284 |
MBON γ2α′1 (3) |
| Genetic reagent (D. melanogaster) | MB080C- split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135128 RRID:BDSC_68285 |
MBON α2sc (2) |
| Genetic reagent (D. melanogaster) | MB082C- split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135130 RRID:BDSC_68286 |
MBON α′2 (3), α3 (5) |
| Genetic reagent (D. melanogaster) | MB083C- split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135131 RRID:BDSC_68287 |
MBON γ3 (5), γ3β′1 (5) |
| Genetic reagent (D. melanogaster) | MB093C- split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135141 RRID:BDSC_68289 |
MBON α′2 (4) |
| Genetic reagent (D. melanogaster) | MB110C-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135158 RRID:BDSC_68262 |
MBON γ3 (5), γ3β′1 (5) |
| Genetic reagent (D. melanogaster) | MB210B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135258 RRID:BDSC_68272 |
MBON γ5β′2a (1), β′2mp (4), β2β′2a (3) |
| Genetic reagent (D. melanogaster) | MB298B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135346 RRID:BDSC_68309 |
MBON γ4>γ1γ2 (4) |
| Genetic reagent (D. melanogaster) | MB310C-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2135358 RRID:BDSC_68313 |
MBON α1 (5) |
| Genetic reagent (D. melanogaster) | MB399B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2501738 RRID:BDSC_68369 |
MBON β2β′2a (2) |
| Genetic reagent (D. melanogaster) | MB433B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2501774 RRID:BDSC_68324 |
MBON β1>α (3), γ4>γ1γ2 (4) |
| Genetic reagent (D. melanogaster) | MB434B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2501775 RRID:BDSC_68325 |
MBON β1>α (4), γ4>γ1γ2 (4) |
| Genetic reagent (D. melanogaster) | MB542B-split-GAL4 | Aso et al., 2014a | FlyLight Robot ID: 2501887 RRID:BDSC_68372 |
MBON α′1 (1), α′3 m (2), α2p3p (2) |
| Genetic reagent (D. melanogaster) | GMR47H09-LexA | Pfeiffer et al., 2013 | FLY: FBtp0088666 RRID:BDSC_53482 |
|
| Genetic reagent (D. melanogaster) | VT055139-LexA | Tirian and Dickson, 2017 | N/A | |
| Genetic reagent (D. melanogaster) | VT018476-lexA | Bidaye et al., 2014 | N/A | |
| Genetic reagent (D. melanogaster) | SS01308-split GAL4 | Janelia Research Campus | N/A | MBON γ5β′2a |
| Genetic reagent (D. melanogaster) | SS01143-split GAL4 | Janelia Research Campus | N/A | MBON β′2mp |
| Genetic reagent (D. melanogaster) | SS1194-split GAL4 | Janelia Research Campus | N/A | MBON α2sc |
| Genetic reagent (D. melanogaster) | SS32219-split GAL4 | Janelia Research Campus | N/A | Lateral Accessory Lobe |
| Genetic reagent (D. melanogaster) | SS32230-split GAL4 | Janelia Research Campus | N/A | Lateral Accessory Lobe |
| Antibody | α-GFP (Rabbit polyclonal) | Life Tech | Cat #A11122 RRID:AB_221569 |
(1:1000) |
| Antibody | α-HA (Rat monoclonal) | Roche | Cat #11867423001 RRID:AB_390918 |
(1:100) |
| Antibody | α-GFP (Chicken polyclonal) | Clontech | Cat #ab13970 RRID:AB_300798 |
(1:2000) |
| Antibody | α-DS (Rabbit monoclonal) | Clontech | Cat #632496 RRID:AB_10013483 |
(1:1000) |
| Antibody | α-CD2 (Mouse monoclonal) | Bio-Rad | Cat #MCA154GA RRID:AB_566608 |
(1:100) |
| Antibody | α-TH (Mouse monoclonal) | Immunostar | Cat #22941 RRID:AB_572268 |
(1:500) |
| Antibody | Goat α-Mouse AF647 (polyclonal) | Thermo Fisher | Cat #A21235 RRID:AB_2535804 |
(1:1000) |
| Antibody | Goat α-Rabbit AF488 (polyclonal) |
Life Tech | Cat #A11034 RRID:AB_2576217 |
(1:400) |
| Antibody | Goat α-Rat AF568 (polyclonal) | Life Tech | Cat #A11077 RRID:AB_2534121 |
(1:400) |
| Antibody | Goat α-Chicken AF488 (polyclonal) |
Life Tech | Cat #A11039 RRID:AB_2534096 |
(1:400) |
| Antibody | Goat α-Rabbit AF568 (polyclonal) |
Life Tech | Cat #A11011 RRID:AB_143157 |
(1:400) |
| Software | Adobe Illustrator CC | Adobe | RRID:SCR_014199 | |
| Software | ZEN | Carl Zeiss Microscopy | Version 2.1 (blue edition) RRID:SCR_013672 |
|
| Software | Fiji | http://fiji.sc | RRID:SCR_002285 |