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Zoom in of a U2OS cell expressing LifeAct-TagGFP2 (gray) and mApple-vinculin (magenta), migrating on a fibronectin (10 µg/ml)-coated high-precision imaging dish. Related to Figure 2A. Time-lapse ring-TIRFM video was recorded with Deltavision OMX SR with 30 s imaging interval. Playback rate 10 frames/s. Scale bar 2 µm.
