Video 5. Non-muscle myosin II (NMII)A pulses assemble into cortical stress fibers under the nucleus of migrating human osteosarcoma (U2OS) cell.

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Zoom in of a U2OS cell expressing histone H2B-eGFP (gray), mApple-NMIIA (green), and miRFP670-paxillin (magenta), migrating on a fibronectin (10 µg/ml)-coated high-precision imaging dish. Related to Figure 3—figure supplement 1A. Time-lapse ring-TIRFM video was recorded with Deltavision OMX SR with 20 s imaging interval. Playback rate 20 frames/s. Scale bar 5 µm.