Video 7. Non-muscle myosin II (NMII)A pulses at the trailing edge of a migrating human osteosarcoma (U2OS) cells.

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Zoom in of a U2OS cell expressing eGFP-NMIIA (green) and mApple-vinculin (magenta) migrating on a fibronectin (10 µg/ml)-coated high-precision imaging dish. Related to Figure 3D and Figure 4C. Time-lapse ring-TIRFM video was recorded with Deltavision OMX SR with 30 s imaging interval. Playback rate 20 frames/s. Scale bar 5 µm.