Figure 8. Snapshots after 1 ms for binary RNA-protein mixtures at T = 298K, with κ = 0.7 and using effective charges according to Equation 5.
[RNA]=0.493 mM and [protein]=0.350 mM. Orange and blue spheres show RNA and proteins, according to size. Concentrations inside the condensates were [RNA:lysozyme]=20.2:20.2 mM; [RNA:trypsin]=16.5:15.2 mM; [RNA:LDH]=9.6:7.2 mM; [RNA:ADH]=9.5:6.7 mM.
Figure 8—figure supplement 1. Snapshots from CG simulations after 1 ms for binary RNA-protein mixtures at T = 298K, with κ = 0.75 using Equation 6 to obtain effective charges.
[RNA]=0.493 mM and [protein]=0.350 mM. Orange and blue spheres show RNA and proteins, according to size. Concentrations inside the condensates were [RNA:lysozyme]=18.6:18.4 mM; [RNA:trypsin]=15.6:14.8 mM; [RNA:LDH]=8.8:7.2 mM; [RNA:ADH]=8.7:6.6 mM.
Figure 8—figure supplement 2. Concentrations of RNA (A) and proteins (B) in dilute and condensed phases as a function of temperature with κ = 1.17.
rRNA = 1.47 nm, qRNA = −46, [RNA]=0.45 mM, [protein]=0.35 mM. Colors indicate proteins: trypsin (blue), alcohol dehydrogenase (violet), lysozyme (red), lactate dehydrogenase (tan), myoglobin (green), cytochrome C (dark red).
Figure 8—figure supplement 3. Charge distribution on protein surfaces based on amino acid residue types (top; basic: blue, acidic: red, polar: green, hydrophobic: white) and electrostatic potentials calculated via a Poisson-Boltzmann continuum model (bottom) with coloring according to the sign of the potential (positive: blue, negative: red).
