Figure 2.

c-Met is a direct target of miR-206. (A) Complementary sequences of miR-206 binding site in WT c-Met 3′-UTR and MUT c-Met 3′-UTR. (B) Luciferase activity was measured to assess the binding ability of miR-206 on the c-Met 3′-UTR WT or MUT in SW480 cells. (C) Spearman's correlation analysis was performed to determine the correlation between miR-206 and c-Met expression levels in SW480 cells. (D) mRNA and (E) protein expression levels of c-Met in HCT116 and SW480 cells were detected via reverse transcription-quantitative PCR and western blot analyses, respectively. *P<0.05, **P<0.01, ***P<0.001. miR, microRNA; WT, wild-type; UTR, untranslated region; MUT, mutant; NC, negative control.