Figure 2.

CTCs isolation and RNA-ISH analysis of epithelial–mesenchymal transition (EMT) markers in CTCs from HCC patients. (A) Process of CTC isolation and detection by CanPatrol™ CTC-enrichment and in situ hybridisation (ISH). Leukocytes were stained for CD45 (white fluorescence). CTCs were stained for the epithelial markers EpCAM and CK8/18/19 (red fluorescence) and the mesenchymal markers vimentin and twist (green fluorescence). The cells were analysed using a 100× objective. (B, C) CTC counts and percentage of each CTC type in patients treated by (B) AR (91.23%; 52/57) and (C) NAR (92.41%; 73/79).