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Mitochondrial ROS promotes Drp1 phosphorylation and mitochondrial fission in endothelial cells under hypoxia/reoxygenation injury. (a, b) Mitochondrial fission was measured using immunofluorescence. Cardiac endothelial cells (CECs) underwent hypoxia/reoxygenation (H/R) injury. Mito-Q, a mitochondrial antioxidant, was added to the CEC media to neutralize mROS. (c–e) qPCR was performed to analyze the transcription of Drp1, Mff, and Fis1 in CECs under H/R injury. ∗p < 0.05.
