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Antioxidant NAC application affect bone metabolism. (a) TRAP staining shows lower number of osteoclasts after adding NAC in a diet of mice. Arrows indicate active osteoclasts. (b) Immunohistochemistry of Runx2. (c) Immunohistochemistry of OPN. (d) Immunohistochemistry of ALP. (e–h) Quantitative analysis of (a–d), respectively. Data are shown as mean ± SD (n = 5). ∗P < 0.05; ∗∗P < 0.01. Scale bars (a–d) 100 μm.
