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. 2021 Feb 4;2021:8658314. doi: 10.1155/2021/8658314

Figure 2.

Figure 2

GIE suppressed the secretion of (a) proinflammatory cytokines IL-6, (b) TNF-α, and slightly increased (c) anti-inflammatory cytokines IL-10 secretion in LPS plus IFN-γ-induced RAW264.7 cells. The effects of GIE on (d) IL-6 and (e) TNF-α mRNA expression. Cells were pretreated with GIE or DEX for 3 h and then coincubated with LPS plus IFN-γ for 24 h. UN: uninduced cells; IN: untreated LPS plus IFN-γ-induced cells; DEX: cells were pretreated with DEX at 1 μM; GIE (50, 100, 200, and 300): cells were pretreated with GIE at concentrations range of 50, 100, 200, and 300 μg/mL, respectively. The data represent the mean ± S.D. of two independent experiments. One-way ANOVA performed the comparison, and Tukey was used as a post hoc test. The degree of significance was denoted with different letters for the comparison between sample groups. p < 0.05 was considered as statistically significant.