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. 2020 Dec 15;40(6):1147–1161. doi: 10.1038/s41388-020-01593-5

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© The Author(s) 2020, corrected publication 2021

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Fig. 1 — A The frequency of integration breakpoints within the HBV genome (NC_003977) is indicated by bars in 29 cases of tumor samples. Integration sites with different supporting counts are indicated by different colors. Genes encoding HBV pre-surface (blue), surface (purple), polymerase (yellow), core (green), and X (red) protein are also shown. B Two pairs of primers are designed for the detection of full-length and 3′-end deleted X gene. 66F + 100R flanks 360 nucleotides (PCR1), 66F + 154R flanks 462 nucleotides (PCR2). Expression of full-length and 3′-end deleted X gene in 87 HBV-related HCC samples were detected by qRT-PCR. C Expression pattern of both full-length and 3′-end deleted X gene in a panel of hepatoma cell lines was detected by RT-PCR, 18 s served as the internal reference.