Fig. 3.

(a) Images of calcium phosphate (CaP)-coated glass substrates after staining with silver nitrate (AgNO₃) following incubation with (I) basic medium (control), (II) RANKL (alone, 50 ng/ml), (III) RANKL+LXA4 (50 ng/ml), (IV) RANKL+LXA4+Boc-2 (10 μM), or in RAW/PDLC coculture in medium supplemented with (V) basic medium (control), (VI) LPS (alone, 10 μg/ml), and (VII) LPS+LXA4 (50 ng/ml), (VIII) LPS+LXA4+Boc-2 (10 μM). Resorbed pits are shown in white, and intact CaP coating is stained black. Note that LXA4 inhibits RANKL or LPS/PDLC-induced osteoclast resorption of CaP coating, and Boc-2 reversed this effect. Magnification: × 10,000. Scale bar: 200 μm. (b) Quantification of resorption pit area (%) resorbed by osteoclasts obtained following incubation of RAW264.7 with (I) RANKL (alone), and (II) LPS (alone) under the same conditions. Statistical analysis was performed by one-way ANOVA with Tukey post-test, n = 100 *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. Asterisks on top of the columns indicate significant differences from the control