Table 1.
Reagents used to prepare calcium and phosphate stock solutions, as well as CPS. Reagents for the calcium and phosphate stock solutions were dissolved using a 0.05 M Tris buffer solution with a pH adjusted to 7.4 using 1 M HCl, while reagents for the CPS were dissolved in MilliQ water
| Solution | Reagent formula | Molar mass | Molarity (M) |
|---|---|---|---|
| Calcium stock | CaCl2 ● 2H2O | 147.02 | 0.025 |
| NaCl | 58.44 | 1.37 | |
| MgCl2 ● 6H2O | 203.30 | 0.015 | |
| Phosphate stock | NaH2PO4 ● 2H2O | 156.01 | 0.0111 |
| NaHCO3 | 84.01 | 0.042 | |
| CPS | NaH2PO4 ● 2H2O | 156.01 | 0.00225 |
| CaCl2 ● 2H2O | 147.02 | 0.004 | |
| NaCl | 58.44 | 0.14 | |
| Tris | 121.1356 | 0.05 |