Table 2.

Clinical protocol for tooth preparation and Immediate Dentin Sealing

First visit: Preparation of the tooth before impression *Tooth preparation is ready for impression, followed by:
1.1 Apply SE Primer (Clearfil SE Bond, Kuraray), active brushing motion	20 s
1.2 Air suction
1.3 Apply SE Adhesive (Clearfil SE Bond, Kuraray), active brushing motion	10 s
1.4 Air-thin	10 s
1.5 Photo-polymerize	10 s
1.6 Apply flowable resin (GrandIO flow, VOCO, Cuxhaven, Germany)
1.7 Photo-polymerize	40 s
1.8 Apply glycerin gel (K-Y* lubricating gelly, Johnson & Johnson, Sezanne, France)
1.9 Photo-polymerize at buccal, oral and proximal sites	40 s each
1.10 Rinse until clean surface
1.11 Clean enamel outline with a rubberpoint or a bur
1.12 Take impression
Second visit: Preparation of the tooth before luting
2.1 Clean tooth surface ultrasonically or with a scaler
2.2 Silica coat the immediate dentin sealing layer (CoJet®-sand, 3 M ESPE, Neuss, Germany)	2–3 s
2.3 Acid etch the enamel (not the accidently exposed dentin)	30 s
2.4 Rinse	30 s
2.5 Dry
2.6 Apply Silane (ESPE – Sil, 3 M ESPE) on the immediate dentin sealing layer	60 s
2.7 Apply SE Adhesive (Clearfil SE Bond, Kuraray)	10 s
2.8 Apply composite (HFO composite, Micerium S.p.A., Avegno, Italy) onto the tooth
2.9 Place the partial restoration onto the tooth
2.10 Remove excess of cement
2.11 Photo-polymerize	40 s
2.12 Apply of glycerin gel (K-Y* lubricating gelly, Johnson & Johnson)
2.13 Photo-polymerize at buccal, oral and proximal sides	40 s each