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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: J Microsc. 2020 Oct 19;281(3):224–230. doi: 10.1111/jmi.12965

Figure 4. X-y imaging of cells fixed at different time points after seeding on to a glass coverslip in the orthogonal imaging chamber.

Figure 4.

Chromatin was stained with Hoechst (H33342) and F-actin was stained with phalloidin. Yellow arrows mark local spots of F-actin enrichment, and local nuclear irregularity in image at 5’. White arrowheads mark spatial variations in curvature of apical F-actin. Scale bar is 5 μm.