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A. Immunostaining for Albumin (ALB), Collagen IV, ZO-1, MRP2, E-cadherin (E-cad), BSEP, F-actin, MDR3, CYP7A1 and HNF4a in HLO. Nuclei were stained with DAPI (blue). Bars, 50 μm.
B. Transmission electron micrograph of an HLO showing microvilli (V) intra-luminal surface; V: Microvilli, BC: Bile canaliculi-like structure, L: Lumen, N: nuclei. Bars, 10 μm.
