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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Gastroenterology. 2020 Nov 2;160(3):863–874. doi: 10.1053/j.gastro.2020.10.042

Figure 2. Liver CRIg+ macrophages exert profound function clearing intestinal mEVs from circulation.

Figure 2.

(A) The levels of PKH26 red fluorescence, CRIg, and F4/80 in the liver of either NCD WT or CRIgKO mice intravenously injected with PKH26-labeled mEVs. (B) Effect of obesity on liver CRIg+ macrophage population. (C) The abundance of PKH26 red fluorescence in the skeletal muscle and epididymal fat of both NCD WT and CRIgKO mice after tail vein injection of PKH26 mEVs. (D) The abundance of 16s rRNA in the liver of both 4wks HFD WT and CRIgKO mice. (E) Effect of palmitate acid (PA, daily 0.8g PA/mouse, 2 weeks) treatment on the liver CRIg+ macrophage population of NCD WT mice. (F) Effect of PA (500 μM) treatment on the apoptosis of liver CRIg+F4/80+ cells. Data are presented as mean ± SEM. n=4 per group (B, E, and F). (A, C, and D) scale bar=50 μm. *P<0.05, **P<0.01, ***P<0.001, Student’s t test.