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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Gastroenterology. 2020 Oct 29;160(3):781–796. doi: 10.1053/j.gastro.2020.10.036

Figure 3. Late treatment with anti-PD-1 is effective when administered in combination with chemotherapy.

Figure 3.

(A) Experimental scheme for late treatment.

(B-C) Gross images (B) and tumor area measured (C) from GAS-KO mice in indicated treatment groups (n = 12-17/group). Dotted lines indicate tumor area. One-way ANOVA (C).

(D) H&E stains of GAS-KO tumors in different treatment groups. Dashed boxes indicate magnified areas shown at the bottom.

(E-G) Immunostaining for cleaved caspase-3 (E), Ki-67 (F) and β-catenin (G) on treated GAS-KO tumors and quantification (n = 3/group). Arrowheads indicate nuclear β-catenin (G). Student’s t-test.

Scale bars, 5 mm (B); 100 μm (D-G). Mean ± SEM. *P < .05; **P < .01; ***P < .001; ****P < .0001.