Skip to main content
. Author manuscript; available in PMC: 2022 Feb 8.
Published in final edited form as: Curr Biol. 2020 Nov 23;31(3):540–554.e5. doi: 10.1016/j.cub.2020.10.071

Figure 5. Contribution of the C-Terminal Domain to the Function of SKIP in Various KO Cell Lines.

Figure 5

(A-E) Confocal immunofluorescence microscopy of Myc-SKIP and Myc-SKIP1–603 expressed by transfection in WT HeLa cells (A), and in HeLa cells with KO of KIF5B (B), KIF1B (C), ARL8B

(D) and ARL8A-ARL8B (ARL8 KO) (E). Untransfected cells were used as controls. Cells were immunostained with antibodies to the Myc epitope (green) and the lysosomal marker LAMTOR4 (red), and counterstained with DAPI (blue). Single channels are shown in grayscale with nuclei in blue. Cell edges are outlined. Scale bars: 10 μm.

(F) Quantification of the percentage of cells displaying the indicated phenotypes in two independent experiments such as those shown in panels A-E. The total number of cells examined in both experiments is indicated on top of each bar.

The related Figure S3 shows the localization of different Myc-SKIP constructs and their effect on lysosome positioning in ARL8-KO cells.