Fig. 3.

Internalization and APCs activation of CM@CaPyro NGs in vitro. (A, B) Uptake kinetics of CM@CaPyro NGs by DC 2.4 and BMDCs. (n = 3; mean ± SEM). (C) Confocal evaluation of free cell membrane and CM@CaPyro NGs colocalization with lysosomes at 6 h after incubation. Cell membrane and nanogranules labeled by NBD-PE (Green). C57BL/6 mice were subcutaneously injected with nanovaccine. (n = 3). (D, E) CD11c, CD86 and CD80 antibodies to characterize the maturation of BMDCs in vitro (n = 4). (F) TNF-α induced by BMDCs was measured using ELISA (n = 3). (G) Levels of antigen presentation on SIINFEKL/H-2Kb in BMDCs induced by cell membrane and CM@CaPyro NGs (n = 4). Data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.