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. 2021 Feb 11;12:939. doi: 10.1038/s41467-021-21184-6

Fig. 2. Design and characterisation of the asymmetric heterodimeric CCPO bipyramidal cage complex.

Fig. 2

a Topological schemes of SBP2 and SBP16; CC pairs are represented as coloured helices. b CD spectra of the proteins SBP2, SBP16 and the complex SBP162 resulting from their interaction (cyan, orange and black, respectively) at 20 °C. c CD signal at 222 nm of the proteins SBP2, SBP16 and the complex SBP162 (cyan, orange and black, respectively) during thermal denaturation, the melting temperatures (Tm) are indicated in the panel. d ITC trace obtained by titrating SBP16 with SBP2 fitted to a 1:1 binding model (Kd = 4.7 ± 0.7 nM). e SAXS experimental profile of the single-chain BIP18SN protein, the complex SBP162 and the subunit SBP16 (grey, black and orange traces, respectively). Error bars in grey represent the standard deviation for each data point (mean). f Vr matrix comparing SAXS profiles obtained for the single-chain BIP18SN protein, the complex SBP162 and the subunit SBP16. Source data are provided as a Source Data file.