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. 2021 Jan 29;11:615868. doi: 10.3389/fimmu.2020.615868

Figure 3.

Figure 3

Peyer’s patch B cells provide the environment for Treg-of-B (P) cell generation with the phosphorylated STAT6 and LAG3 expression. To determine whether Peyer’s patch B cells are important for Treg-of-B (P) cells generation through STAT6-LAG3 pathway, CD25- CD4+ T cells were cultured with or without Peyer’s patch B cells in presence of anti-CD3 plus anti-CD28 1 µg/ml for 3 days. CD4+ cells were gated to represent Treg-of-B (P) cells in B-T cocultured. Phosphor-STAT6 and LAG3 (A) and the Annexin V (B) expressed by T cells or Treg-of-B (P) cells were analyzed by flow cytometry. (C) After three day cocultured, Peyer’s patch B cells were depleted and T cells were harvested to determine the suppressive function. Red line, T cells cultured with B cells (+B); black line, T cells cultured without B cells (-B); gray line, isotype control. Results are expressed as the mean ± SEM. Data are representative of four different experiments. **p < 0.01, ***p < 0.005, compared with the -B group or responder T cell group. ###p < 0.005, compared +B group.