Table 1.
Biochemical analysis and nutritional composition of the pelleted feed used as food for the Lates calcarifer Seabass during the experiments.
| Ingredients (%) | g 100 g-1 diet (dry) |
|---|---|
| Fish meal | 45.00 |
| Shrimp meal | 6.50 |
| Soybean meal | 24.00 |
| Octopus,Squid meal | 4.40 |
| Bread flour | 5.00 |
| Rice bran | 3.40 |
| Cod liver oil | 5.40 |
| Vitamin and mineral premix1 | 5.00 |
| Fish protein Hydrolysate | 1.30 |
| Total | 100 |
| Biochemical composition1, 2, 3, 4(%) | |
| Moisture | 10.8 ± 0.4 |
| Crude protein3 | 44.8 ± 0.5 |
| Crude fiber | 9.1 ± 0.5 |
| Crude lipid | 10.5 ± 0.06 |
| Ash | 9.3 ± 0.5 |
| NFE 4 | 26.3 ± 0.8 |
| Energy (kJg-1) | 20.93 ± 0.6 |
Vitamin and mineral composition contained the following ingredients (g 100 g-1 diet): Hexuronic acid (vit C), 16.2; thiamin HCl (vit B6)), 1.2; inositol, 38.2; calcium, 1.05; zinc, 1.1;choline chloride, 4.0; retinol (vit A), 1.2; phosphorus, 2.9;magnesium, 3.0; copper, 1.1; pyridoxine (vit B6), 1.2; α-tocopherol acetate (vit E), 4.8; phospholipids, 3.7; folic acid, 0.6;riboflavin (vit B2), 1.3; cholecalciferol (vit D3), 6.4; menadione sodium bisulfate (vit K3), 0.04; cyanocobalamin (vit B12), 0.008 manganese, 1.0; iodine, 1.5; sodium, 2.0; iron, 1.1; biotin, 0.65; nicotinic acid, 3.4;
Dry matter basis (%): mean ± SE, number of determination = 5
Measured as nitrogen × 6.25.
Nitrogen-free extract = 100 – (% protein + % fat + % ash + % fiber).