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. 2021 Feb 11;11:3551. doi: 10.1038/s41598-021-82893-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Human antibody responses to recombinant F2-P27, F0 and F2-derived synthetic peptides. Shown are IgG (a), IgA (b) and IgM (c) levels (y-axis: optical density values) in sera from 23 adult individuals to recombinant F2-P27, F0 and F2-derived synthetic peptides (P1–P6), (x-axis). Horizontal lines within scatter plots indicate median values. The cut-off (mean of buffer control plus three times standard deviation) is indicated by a red line. Significant differences of antibody responses are indicated: ns (not significant); *p < 0.05; **p < 0.001; ***p < 0.0001.