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. 2021 Feb 11;12:942. doi: 10.1038/s41467-021-21195-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b Colony forming units (CFU) count in larvae reared in water collected from Ae. aegypti breeding sites for 48 h (a) or 72 h (b) after 2, 5 and 24 h from transferring into sterile rearing water. Bacterial loads of non-transferred larvae are shown in black, while those of transferred larvae are shown in grey. c, d Development success of larvae continuously reared in breeding site water or transferred into sterile rearing water after 48 h (c) or 72 h (d) expressed as percentage of individuals reaching adulthood (striped bars), dying (black bars) and with stalled development (grey bars). In (a–d), data show mean ± SEM of two (a), three (b, c) or seven (d) independent replicates. The exact number of individuals analysed per condition and replicate is indicated in each panel. Statistical significance was determined with generalised linear mixed models and least square means with Bonferroni correction. Exact p values are indicated in the figure. See Table S1 for detailed statistical information. Source Data are provided as a Source Data file.