Figure 2.

Microglia Co-culture Increased Expression of Dopaminergic and Midbrain-Specific Markers

(A) qRT-PCR data for the plasma membrane DAT, VMAT2, the midbrain-specific transcription factors PITX3, the homeobox protein EN1, AADC, and the midbrain dopaminergic LMX1A upon co-culture differentiation of hVM1-Bcl-X_L NSCs and BV2 microglia. Student's t test. Control, n = 4, N = 2; co-culture, n = 4, N = 2. Mean ± SEM. ^∗p < 0.05, ^∗∗p < 0.01, ^∗∗∗p < 0.001.

(B) Expression of the floorplate marker FOXA2 in TH⁺ neurons. Scale bar: 100 μm.