Microglial Cells Treated with LPS and IL-4 show Pro- and Anti-inflammatory Activation Profiles, Respectively
(A) BV2 microglia were activated with 100 ng/mL LPS or 20 ng/mL IL-4 for 24 h prior to co-culture with hVM1-Bcl-XL cells.
(B) Immunocytochemical staining of activated BV2 microglia for the microglial marker Iba1. Scale bar: 100 μm.
(C) Cytokine profiling of microglia medium after 24 h of activation. Untreated, n = 5, N = 3; LPS, n = 7, N = 4; IL-4, n = 7, N = 4.
(D) ELISA for IGF1 of microglia medium at 24 h post activation. Untreated, n = 4, N = 3; LPS, n = 6, N = 4; IL-4, n = 6, N = 4. One-way ANOVA, Tukey's multiple comparison test. Mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.
See Figures S5 and S7 for additional data.