Figure 3.

F/A-primed Pluripotent Cells Fail to Generate Gastruloids

(A) Schematic representation of the experimental design. F/A, FGF/ACTIVIN A.

(B) Representative bright-field images of 2i + LIF ESCs, EpiLCs, and EpiSC-derived aggregates at 48 h (left) and boxplot diagram (right) of the distribution of the diameters. Red arrows indicate detached cells and debris (n = 3; 30 gastruloids/condition; bar, 100 μm;^∗p < 0.01).

(C) Representative bright-field images of the gastruloids and aggregates derived from 2i + LIF ESCs, EpiLCs, and EpiSCs, respectively. Data are expressed as mean ± SD (n = 3; 60 gastruloids/organoids/condition) (bar, 50 μm).

(D) Representative bright-field images (left) of 2i + LIF ESC-, EpiSC p0-, and EpiSC p6-derived aggregates/gastruloid-like organoids at the indicated time points, and quantification (right) of the elongated gastruloids at 120 h. Data are expressed as mean ± SD (n = 3; 60 gastruloids/condition; bar, 100 μm; ^∗p < 0.01).

(E) Representative bright-field images (left) of gastruloids/organoids derived from 2i + LIF- and F/A-treated cells at the indicated time points (bar, 200 μm) and quantification of percentage (right) of elongated gastruloids. Data are expressed as mean ± SD (n = 3; 60 gastruloids/condition; ^∗p < 0.01).

See also Figure S3.