Figure 4.

Gastruloid Aggregation Kinetics

(A) Representative bright-field images (left) of aggregates derived from 2i + LIF ESCs, EpiLCs, and EpiSCs p0 at the indicated time points (bar, 100 μm). Red arrows indicate detached cells. Time course quantification (right) of diameters of 2i + LIF ESC-, EpiLC-, and EpiSC p0-derived aggregates. Data are expressed as mean ± SD (n = 3; 30 gastruloids/condition; ^∗p < 0.01).

(B) Pie chart quantification of differentially expressed genes (left) and KEGG pathway enrichment (right) of upregulated genes in EpiSCs p0 versus 2i + LIF.