Figure 7.

Primordial Germ Cell-like Cell (PGCLC) Competence Discriminates Different Pluripotent States

(A) Schematic representation of the experimental strategy.

(B) Representative bright-field images of 2i + LIF-, PiC-, and EpiLC-derived aggregates (96 h) obtained as described in (A) (bar, 100 μm).

(C) Representative confocal images of cytospun cells from 2i + LIF-, PiC-, and EpiLC-derived aggregates (96 h) double stained with OCT4 (red) and BLIMP1 (green). Nuclei were counterstained with DAPI (bar, 100 μm).

(D) Quantitative real-time PCR analysis of Oct3/4, Blimp1, Prdm14, and Nanos3 gene expression under the indicated conditions. Data represent fold change versus day 0; data are normalized to Gapdh and are mean ± SD (n = 3; ^∗p < 0.01).

(E) Representative confocal images (left) and quantification (right) of AP2-γ-positive cells under indicated conditions (right). Nuclei were counterstained with DAPI. Data are mean ± SD (n = 3; ^∗p < 0.01) (bar, 100 μm).