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. 2021 Feb 11;9:13. doi: 10.1038/s41413-020-00130-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Spreading behavior of BMSCs on different SF substrates. a Representative mosaic immunofluorescence images of vinculin (purple), F-actin (white), and nuclei (blue) in BMSCs without and with CKB treatment and the corresponding orientation plots for F-actin staining, where the different colors indicate different orientations of actin filaments as per the given color map. b–e Quantitative analysis of (b) FA area per cell, (c) cell shape index, (d) cell area, and (e) F-actin anisotropy in BMSCs. f–h Single-cell scatter plots of F-actin anisotropy as a function of (f) cell area, (g) FA area per cell, and (h) cell shape index. i SEM images of BMSCs’ spreading morphology. j–m The mRNA expression levels of (j) Vinculin, (k) Vinexin α, (l) Cap, and (m) Rhoa in BMSCs. For each box plot, the box boundaries represent the 25th–75th percentiles, and the whiskers represent the min and max values. The central line and cross represent the median value and mean, respectively. Error bars represent one standard deviation. (*P < 0.05 and **P < 0.01)