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. 2021 Feb 11;11:3639. doi: 10.1038/s41598-021-83058-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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URDV density per Kb coding sequence of NTD candidate genes. The majority of 302 NTD candidate genes show URDV/KbCDS density less than one. The x-axis showed the gene names ordered by the URDV/Kb CDS from highest (4.1 for CTNNBP1) to lowest (1.28, CYP26C1). Dashed line (- - - -) showed the URDV/Kb CDS ratio of the gene and dotted line (…….) showed the length of CDS in Kb. Count of URDV in EA showed in blue bar and MA showed in red bar. Approximately half of the 64 genes had URDVs found in both EA and MA. One-third of the 64 genes have ≥ 2 URDVs/Kbp CDS.