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. 2021 Feb 11;12:945. doi: 10.1038/s41467-021-20929-7

Fig. 2. The acinus pinin double mutants showed ABA hypersensitive phenotypes.

Fig. 2

a, b Germination rates of WT, acinus-2, pinin-1, and acinus-2 pinin-1 after different days on ½ MS medium without ABA (a) or with 0.25 μmol/L ABA (b). The data points of WT, acinus-2, and pinin-1 overlap. Statistically significant differences between WT and acinus-2 pinin-1 were determined by two-tailed t test. The P values for a and b in a are 1.24E−5 and 2.96E−2. The P values for a, b, c, d, e, f, g, h, and i in b are 7.69E−3, 5.60E−6, 2.88E−6, 3.92E−4, 3.17E−4, 6.00E−3, 3.10E−2, 4.77E−2, and 8.57E−3. c Seed germination rates of the indicated genotypes on ½ MS medium supplemented with increasing concentrations of ABA after 5 days. Note that the data points of acinus-1 pinin-1 and acinus-2 pinin-1 overlap and those of WT, acinus-1, acinus-2, and pinin-1 overlap. Statistically significant differences between WT and acinus-2 pinin-1 were determined by two-tailed t test. The P values for a, b, and c are 3.51E−2, 5.14E−8, and 2.99E−2. d Seed germination and development of the indicated genotypes on ½ MS medium with or without 0.5 μmol/L ABA. The pictures were taken 6 days after germination. Values represent mean ± SD calculated from three biological replicates (n = 3) for a–c.