Figure 3.

(A) Fluorescence microscopy of VX2 tumor cells with Live/Dead staining showing more dead cells (red) and less viable cells (green) in the combination treatment of DOX plus 42°C hyperthermia compared to other control groups of DOX alone, 42°C hyperthermia alone, and PBS. MTS assay demonstrating the lowest cell viability in the combination therapy of DOX plus 42°C hyperthermia compared to other treatments (***p<0.001). (B) Flow cytometry showing the combination treatment with DOX plus 42°C hyperthermia induced early apoptosis of VX2 cells compared with other treatments (***p<0.001).