Figure 4.

Structural superimposition and electrostatic surface comparison of SARS-CoV-2 ORF7a to the crystal structure of ICAM3–LFA-1 complex

(A) Structural superimposition of SARS-CoV-2 ORF7a with the ICAM3 in the crystal structure of the ICAM3–LFA-1 complex.

(B) Electrostatic surface comparison of SARS-CoV-2 ORF7a with the crystal structure of ICAM3–LFA-1 complex. The ICAM3–LFA-1 complex is split with the interaction interfaces from a front view perspective. The electrostatic surface of SARS-CoV-2 ORF7a was generated based on its structural superimposition to ICAM3.

(C) View of the detailed interactions of the varied residues (Lys72) in SARS-CoV-2 ORF7a with the nonpolar residues (Leu204 and Leu205) in LFA-1.

(D) Detailed interaction view of the varied residues (Gln62) in SARS-CoV-2 ORF7a with the nonpolar residues (Met140) in LFA-1. Dot representation indicates steric occupation of the residues. LFA-1 is in brown, ICAM3 is in red, and Mg²⁺ ions are in purple.

See also Figures S2–S6 and Table S2.