Fig. 5.

miRNA-451a inhibits Th2 cell differentiation through downregulating ETS1. Cells overexpressing miRNA-451a were infected with LV-OE-ETS1 or LV-OE-NC. a Representative images of flow cytometry analysis showing GATA3+CD4+ cells. b The mRNA levels of IL5 and IL13 were analyzed by qRT-PCR. c The protein expression of ETS1, IL5, and IL13 were determined with Western blot. d ELISA analysis of IL5 and IL13. The quantitative analyses in b and c are presented as the mean ± SD calculated based on three separate experiments with two-tailed unpaired t tests (n = 3). ** p < 0.01, *** p < 0.001. NC, negative control; OE, overexpression.