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. 2021 Feb 10;220(4):e201912060. doi: 10.1083/jcb.201912060

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© 2021 Chang et al.

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Figure S1. — Interactome analysis of TORC1. (A) Impaired TORC1 activity by C-terminally tagging to Tor1 or Kog1. (B) Localization of overexpressed TORC1 at the vacuolar membrane. o/e indicates overexpression under the HXK2 promoter. Scale bars, 1 µm. (C) CoIP assay of Kog1 interactors. C-terminally Myc-tagged proteins were immunoprecipitated, and coimmunoprecipitated GFP-Kog1 was detected. GFP-Kog1 was expressed under the HXK2 promoter. The coIP assay for seven interactors is shown in Fig. 1 D.