Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 10;220(3):e202003173. doi: 10.1083/jcb.202003173

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Audano et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 1. — Zc3h10 controls white adipogenesis and pre-mRNA transcription in preadipocytes. (A and D) Lipid droplets were labeled by using AdipoRed dye to evaluate lipid accumulation in mature adipocytes (day 9). Scale bar: 50 µm. (B and E) Relative quantification of AdipoRed-positive (AdipoRed⁺) cells in mature adipocytes (day 9). n = 4; unpaired parametric Student’s t test; *P < 0.05 vs. Scramble; ***P < 0.001 vs. Control. (C and F) Lipid droplet size adipocytes differentiated for 9 d. n > 1,000; unpaired and nonparametric Mann-Whitney test; ***P < 0.001 vs. Scramble/Control. (G and J) Primary stromal vascular cell (SVC) differentiation was evaluated in Scramble and ShZc3h10 mature adipocytes (day 8) by using AdipoRed dye. Scale bar: 50 µm. (H and K) AdipoRed⁺ cell counts in Scramble and ShZc3h10 primary adipocytes (day 8). n = 3; unpaired parametric Student’s t test; ***P < 0.001 vs. Scramble. (I and L) Lipid droplet size in primary mature adipocytes (day 8). n > 3,300 ShZc3h10; n > 3,500 Zc3h10 Flag; unpaired nonparametric Mann-Whitney test; ***P < 0.001 vs. Control. (M) Western blot of Zc3h10 in whole-cell lysates during the first 48 h of differentiation. (N) Western blot of Zc3h10, Nup98 (nuclear marker), α-tubulin (cytoplasmic marker), and histone H3 (chromatin marker) in the indicated cell fractions 36 h after differentiation induction. (O) Volcano and box plots of downregulated (red), upregulated (blue), and unchanged (gray) pre-mRNAs in ShZc3h10 preadipocytes at 36 h. Scramble: n = 2; ShZc3h10: n = 3; volcano plot: false discovery rate (FDR) < 0.05; box plot: Kruskal-Wallis test of rank with Dunn’s post-hoc test; ***P < 0.001 vs. Scramble. (P) GAUGE analysis results of significantly down- and upregulated pre-mRNA clusters in ShZc3h10 36-h-differentiated preadipocytes. Table indicates pathway name, trend, normalized enrichment score (NES), number of differentially expressed genes (DEGs) along with their percentage of the total genes in the cluster, and adjusted P value (padj). (Q) Volcano plot of the actin filament–based process cluster genes. Red dots represent DEGs that were significantly downregulated (FDR < 0.05) in ShZc3h10 cells 36 h after differentiation induction. (R) Volcano plot of the translation gene cluster. Genes that were significantly upregulated (FDR < 0.05) in ShZc3h10 preadipocytes 36 h after differentiation induction are shown in blue.