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. 2021 Feb 10;220(3):e202003173. doi: 10.1083/jcb.202003173

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© 2021 Audano et al.

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Figure 2. — The Zc3h10 transcriptomic signature is associated with F-actin dynamics in preadipocytes. (A and C) Left: Representative confocal images of F-actin detected by using the SiR-actin probe in preadipocytes (36 h). Middle and right: Enlarged images (middle) and relative binary images (right) are also shown. Scale bar: 50 µm (left), 10 µm (middle and right). (B and D) Quantitative analysis of the F-actin networks preadipocytes (36 h). n = 6 ShZc3h10; n = 7 Zc3h10-Flag; unpaired parametric Student’s t tests; *P < 0.05 vs. Scramble (B); *P < 0.05 (D); ***P < 0.001 vs. Control. (E and F) Representative confocal images of F-actin (green) and lipid droplets (red), as well as F-actin binary images of preadipocytes on day 5 after differentiation. Scale bar: 50 µm.