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. 2021 Feb 12;19:21. doi: 10.1186/s12958-021-00701-8

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Expression levels of predicted target genes in the trophoblasts after the transfection with miRNA-4497 agomir. a Levels of miRNA-4497 and mRNAs of 12 predicted target genes measured by real-time PCR after the transfection of HTR-8/SVneo cells with miRNA-4497 agomir or scrambled sequences used as a negative control (NC). Note the significant reduction in SP1, CXCR5 and BMP8A mRNA levels after overexpression of miRNA-4497. b The protein expression levels of SP1, CXCR5 and BMP8A determined by Western blotting after transfection of BeWo and HTR-8/SVneo trophoblast cell lines with miRNA-4497 agomir or negative control (NC). Note the significant decrease in the protein levels of SP1 and CXCR5 after overexpression of miRNA-4497. *, P<0.05; **, P<0.01; ***, P<0.001