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. 2021 Feb 11;21:15. doi: 10.1186/s12896-021-00669-8

Fig. 1.

Fig. 1

Schematic of CRISPR/Cpf1 in a single-plasmid system for use in fungal genome engineering application. In this study, FnCpf1 and crRNA are expressed under A. nidulans TEF1 Pol II promoter and A. fumigatus U3 Pol III promoter, respectively, to edit genome of the wild-type strain of A. aculeatus TBRC 277