. 2021 Feb 11;21:15. doi: 10.1186/s12896-021-00669-8

Table 1.

Plasmids and fungal strain used in this study

Plasmids/Strain	Genotype	Reference
Plasmid
pFC902	Amp^R, pyrG, AMA1, U3-AF crRNA Cas9	[21]
pJET1.2B-Km^R	Amp^R, kan^R	This study
pJET-U3P sgRNA	Amp^R, U3-AF sgRNA Cas9	This study
pFC333	Amp^R, ble^R, AMA1, TEF-Cas9	[22]
pCRISPR0	Amp^R, ble^R, AMA1	This study
pCRISPR01	Amp^R, ble^R, kan^R, AMA1	This study
pCRISPR01-eGFP	Amp^R, ble^R, kan^R, AMA1, TEF1-EGFP	This study
pCRISPR01-FnCpf1	Amp^R, ble^R, kan^R, AMA1, TEF1-FnCpf1	This study
pCRISPR01-FnCpf1-eGFP	Amp^R, ble^R, kan^R, AMA1, TEF1-Cpf1-EGFP fusion	This study
pOK-U3P crRNA pyrG-1	Kan^R, U3-AF crRNA-pyrG-1	This study
pOK-U3P crRNA pyrG-2	Kan^R, U3-AF crRNA-pyrG-2	This study
pOK-U3P crRNA pyrG-3	Kan^R, U3-AF crRNA-pyrG-3	This study
pCRISPR01-FnCpf1-pyrG-1	Amp^R, ble^R, kan^R, AMA1, TEF-Cpf1, crRNA-pyrG-1	This study
pCRISPR01-FnCpf1-pyrG-2	Amp^R, ble^R, kan^R, AMA1, TEF-Cpf1, crRNA-pyrG-2	This study
pCRISPR01-FnCpf1-pyrG-3	Amp^R, ble^R, kan^R, AMA1, TEF-Cpf1, crRNA-pyrG-3	This study
Strain
A. aculeatus TBRC 277	Wild type	[1]