FIGURE 5.

Effect of SAAP-148 on MRSA persisters in antibiotics-exposed mature biofilms on TAN disks. Bacteria obtained by sonication from antibiotics-exposed mature biofilms on TAN disks were exposed for 2 h to SAAP-148. This resulted in eradiation of the bacteria at SAAP-148 doses of ≥1.6 μM (A). SAAP-148 dose-dependently reduced bacterial counts in intact biofilms (without sonication) on TAN disks at doses ≥51.2 μM (B). Prolonging the exposure of the biofilms to SAAP 148 to 24 h resulted in similar eradication of bacteria at doses ≥51.2 μM (C). Addition of SAAP 148 for 24 h during an additional fourth day of antibiotic exposure on the intact biofilm also resulted in eradication of bacteria (D). Solid lines denote the median log CFU/mL (n = 3 experiments, each in duplicate).