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Phosphorylation of DBN3a NS5A. Lysates of Huh7 or Huh7.5 cells stably harbouring either SGR-feo-JFH-1 or SGR-neo-DBN3a were probed using a sheep polyclonal antiserum to detect total NS5A (red), together with phospho-specific antibodies to pS225 (a) or pS232 (b) (green signal). NS5A species are identified by either red dots (basally phosphorylated) or green dots (hyper-phosphorylated). Naïve Huh7.5 cells were included as a control.
