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. 2020 Sep 8;101(11):1182–1190. doi: 10.1099/jgv.0.001486

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© 2020 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.

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Fig. 5. — Replication of SGR-luc-DBN3a with resistance-associated substitutions (RAS). Huh7.5 cells were electroporated with in vitro-transcribed SGR-luc-DBN3a RNA with either the RAS Y93H or an in-frame deletion of proline 32 (ΔP32). Luciferase levels were measured at the indicated times and compared to mock, original and NS5B-GNN controls. n=3, error bars represent sem. **P <0.01, ***P <0.001 for 72 h p.e. values compared to original.