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. Author manuscript; available in PMC: 2021 Feb 12.
Published in final edited form as: Science. 2020 Jul 10;369(6500):167–173. doi: 10.1126/science.aaw2622

Fig. 4. Increased systemic Gpld1 alters signaling cascades downstream of GPI-anchored substrate cleavage in the aging systemic milieu.

Fig. 4.

(A) Heat maps of top 20 proteins up- and down-regulated in blood plasma of aged mice after Gpld1 HDTVI relative to GFP HDTVI control, identified by mass spectrometry. (B and C) Western blot with corresponding Ponceau S stain and quantification of plasminogen [Plg; (B)] and vitronectin [Vtn; (C)] in equal volumes of blood plasma from individual aged mice 24 hours after HDTVI of Gpld1 or GFP control (n = 4 per group). (D) List of 41 proteins down-regulated in blood plasma from aged mice after exercise. (E and F) Enrichment analysis of plasma proteins down-regulated with Gpld1 HDTVI (E) or exercise (F) in aged mice, as identified by mass spectrometry. The number of proteins represented in each process is listed to the right of each bar. Data are means ± SEM; **P < 0.01, ***P < 0.001 [t test in (B) and (C)].