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. 2021 Feb 11;22:174. doi: 10.1186/s12891-021-04043-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — A. Showing the PCR amplification band of the positively cloned CBFB/pCDH plasmid vector was 564 bp, which was consistent with the sequence map. B. CBFB/ plkko.1 plasmid vector positive clones were 5 kbp and 2 kbp after double enzymatic digestion, and the sequencing results were consistent. C. Validating Western immunoblot results of plasmid transfection efficiency; D. cell proliferation in each group as detected by the CCK-8 assay; E, F. cellular apoptosis in each group as detected by Annexin V/PI double staining (*: p < 0.05, **: p < 0.01, ***: p < 0.001)